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| Klautau-Guimarães,Maria de Nazare; DAscenção,Renata; Caldart,Fabiana A.; Grisolia,Cesar K.; Souza,Jurandir R. de; Barbosa,Antonio C.; Cordeiro,Célia M.T.; Ferrari,Iris. |
| We investigated Individual differences in susceptibility to methylmercury (MeHg) contamination and its relationship with polymorphisms of the detoxifying enzyme glutathione S-transferase (GST). In Brazil, some Amerindian tribes from the Amazon region have an increased level of mercury in their hair. Samples of hair and blood were taken from inhabitants of two villages in the Kayabi and Munduruku Amerindian communities to investigate mercury levels in association with genetic polymorphism of GSTs. Other molecular biological markers were also studied, such as hemoglobin, haptoglobin and glucose 6-phosphate dehydrogenase (G-6-PDH). Higher levels of mercury contamination were found in the Kayabi villagers, who had a null genotype (GSTM1 0/0, also denominated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amerindian; Genetic polymorphism; Glutathione S-transferase; Mercury. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500027 |
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| Khouri,Juliana; Resck,Inês S.; Poças-Fonseca,Marcio; Sousa,Thiago M.M.; Pereira,Luciana O.; Oliveira,Adriano B.B.; Grisolia,Cesar K.. |
| The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caryocar brasiliense; Anticlastogenicity; Antioxidant; Micronucleus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024 |
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| Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K.. |
| The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025 |
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| Dantas,Alisson F.; Lopes,Renata M.; Fascineli,Maria L.; José,Solange C.B.R.; Pádua,Juliano G.; Gimenes,Marcos A.; Grisolia,Cesar K.. |
| Abstract This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa L.; Phaseolus vulgaris L.; Methyl methanesulfonate; Cytogenetic test; Comet test. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100145 |
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| Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Pereira,Luiz C.S.; Klautau-Guimarães,Maria N.; Grisolia,Cesar K.. |
| Physical training induces beneficial adaptation, whereas exhaustive exercises increase reactive oxygen-species generation, thereby causing oxidative damage in plasma and erythrocytes, fractions susceptible to lipid peroxidation. Pequi (Caryocar brasiliense Camb.) is a Brazilian Cerrado fruit containing a carotenoid-rich oil. The aim was to investigate the effects of pequi-oil on exercise-induced oxidative damage in plasma and erythrocytes, after running in the same environment and undergoing weekly training under the same conditions as to type, intensity and length. Evaluations were accomplished after outdoor running on flat land before and after ingestion of 400 mg pequi-oil capsules for 14 days. Blood samples were taken after running and submitted to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caryocar brasiliense Camb hemogram hematimetric indexes (MCV; MCH; MCHC and RDW) haptoglobin antioxidant enzyme polymorphisms. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200026 |
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| Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Hiragi,Cássia O.; Penalva,Guilherme C.; Oliveira,Silviene F.; Grisolia,Cesar K.; Klautau-Guimarães,Maria N.. |
| Haptoglobin is a plasma hemoglobin-binding protein that limits iron loss during normal erythrocyte turnover and hemolysis, thereby preventing oxidative damage mediated by iron excess in the circulation. Haptoglobin polymorphism in humans, characterized by the Hp*1 and Hp*2 alleles, results in distinct phenotypes known as Hp1-1, Hp2-1 and Hp2-2, whose frequencies vary according to the ethnic origin of the population. The Hp*1 allele has two subtypes, Hp*1F and Hp*1S, that also vary in their frequencies among populations worldwide. In this work, we examined the distribution frequencies of haptoglobin subtypes in three Brazilian population groups of different ethnicities. The haptoglobin genotypes of Kayabi Amerindians (n = 56), Kalunga Afro-descendants (n =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian ethnicities; Haptoglobin; Polymorphism; Subtypes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300005 |
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| Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.. |
| Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis , Cichla temensis , Hoplias malabaricus , Astyanax bimaculatus lacustres , Oreochromis niloticus , Cyprinus carpio and Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in Cichla temensis and Hoplias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021 |
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| Oliveira-Martins,Cynthia R.; Grisolia,Cesar K.. |
| The toxicity and genotoxicity of wastewater from eight gasoline stations in Brasília, Brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in Allium cepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (Oreochromis niloticus). The content of gasoline station effluents was also analyzed based on several physico-chemical parameters. None of the wastewater samples was genotoxic to A. cepa root cells, although cell proliferation was significantly inhibited, especially at the highest concentrations. Likewise, no micronuclei were observed in O. niloticus peripheral erythrocytes, even after exposure to high concentrations, but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; Chromosomal aberrations; Gas station; Micronucleus; Oreochromis niloticus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400029 |
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